Hadi Ostadi

10 Questions 14 Answers 0 Followers

Questions related from Hadi Ostadi

Do anyone help me regarding this matter?

I am currently working on project in order to increase stability of protein against oxidation. In order to remove oxygen in this solution, I have to apply nitrogen gas. I have a half full glass...

06 June 2018 4,448 0 View

Increasing acicd isoform in protein solution

I am currently working on a purification kind of MAb. Its PI is 6.11. in one step of process I have to decrease its pH near 5.2 because of some considerations. The base of buffer is tris-HCL. data...

08 August 2017 2,152 1 View

Elimination of yellow color from final product of protein?

I am currently purifying a protein (hGH-22.13 kDa). At first it was fusion protein and has His-tag and sumo as ubiquitin. The stages of purification are Ni-NTA, G25, digestion to remove His-tag...

08 August 2017 5,922 0 View

Yellowish color of final h GH protein which was purified?

I am currently working on the His SUMO hGH purification process. After the purification of fusion protein, target protein undergoes a digestion stage and is separated from hGH using a SUMO...

05 May 2017 5,916 5 View

Does it correct apprroach to eliminate or degradate endotoxin from protein solution?

could any one help me regarding this question? I'm currently working on the purification of protein process. There is endotoxin in final step of purification process. I applied different methods,...

04 April 2017 3,942 0 View

Creation endotoxin in final sample!!!?

I'm currently working on purification process. In final step of purification (after anion exchange step of purification (q-sepharose)) different fractions were collected. Endotoxin test by use of...

04 April 2017 752 9 View

Capture human EPO to the special IMAC resin?

I'm currently working on the purification of EPO which is secreted from CHO cell . for final step of purification I have to applied IMAC-Fe(III) for an especial goal.(Endotoxin removal) could any...

04 April 2017 8,584 10 View

Cleavage of fusion protein?

I am currently working on purification protein process. For cleavage of target protein from sumo I applied sumoprotease. the activity of enzyme was too low, could any one recommend me an especial...

02 February 2017 5,192 4 View

I want to filtered cell's which is disrupted by high pressure homogenizer can anyone suggest me filtration system or facility instead of centrifuge?

After cell disruption in industrial scale, I want to eliminate cell's which is distributed, I should find some facilities such as filtration system, instead of centrifuge, could anyone please...

09 September 2016 9,994 4 View

Hello, could any one help me about a purification of protein process?

In final steps of protein purification process , I fond out that after equilibration of q sepharsoe resin by this buffer, (20mM tris-HCl, 250 mM glycine, pH 7.4), final pH of resin after passing...

08 August 2016 9,601 6 View