Where can I download an MRI dataset of the human pelvis?

I am looking for a good/high-quality MRI dataset of the human pelvic region. Do you know where I can find this? Thanks!

13 October 2020 7,738 5 View

Has anyone received negative RFU values for one of your replicates in qPCR?

We are running qPCR with SYBRGreen to amplify an inserted 12S ribosomal DNA region of a plasmid. When we ran this amplification for the first time (in duplicate), one replicate looked fine while...

03 April 2018 4,926 4 View

Interpolation of 3D wind data (for every lon,lat,time) based on drifter positions and time (different matrix dimensions)?

Hello everyone, I am working with some meteorological data from SKIRON which I converted from grib to netcdf in order to make the necessary calculations and formatting for interpolation. I want to...

17 January 2018 6,415 3 View

What are the best procedures and techniques of teaching STEM education in the twenty first century?

I am interested in searching for the best procedures and techniques of teaching STEM to motivate and sustain students' interest in STEM particularly chemistry. p

05 September 2017 9,622 3 View

Is there a problem with using a large sample size in your data analysis after you have determined a small sample size before the study?

29 May 2017 6,595 10 View

What is the minimum soil sample size (in g) that should be used when wet sieving to examine mycorrhizal fungi spore abundance and/or diversity?

10 July 2016 7,749 11 View

Urgent: does this analysis of a repeated measure manova make sense?

background: 100 students were given an unrelated text and a related text each. they then had to answer questions on each text, say how interested they were in each text, and then evaluate how much...

30 May 2016 6,467 3 View

How can we confirm transfection of a WT gene into a cell line?

We are transfecting mutations and matched WT genes. We'll be using antibiotic selection and have generated kill curves to determine the optimal concentration of the selectable marker in the cells...

19 May 2015 4,820 8 View

What other factors can affect failure of hydrogels to form?

06 April 2015 7,411 4 View

Are you familiar with a freeze thaw method to make hydrogels?

I am currently employing the freeze thaw method to synthesize 10% w/v polvinyl alcohol. However, I cannot get the solution to gel. It becomes a sticky paste instead. I am only using a refrigerator...

20 March 2015 5,015 9 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View