There are H5N1 viruses which replicate to higher HA titer in SPF eggs. Does that mean those viruses are more adapted to poultry birds?
Any reference would be acknowledged.
Thanks
I believe that the answer for your question is "yes"; but look at the positive aspect in this issue. The egg-adapted high-growth (PR8) virus strain has been widely used as a master donor virus to generate vaccine seed viruses for manufacturing egg-based inactivated influenza vaccines including the H5N1 viruses.
Honestly a better empirical test than just titers would be to do a simple longitudinal growth kinetics in replicate eggs with proper negative controls over set time-points (0h, 2h, 4h, 8h, 12h, then 1d, 2d, 3d, 4d, 5d or something like that) and look at starting point of titer recovered at like 30 min p.i. (maybe there is an infection/entry advantage but kinetics are otherwise the same), timing/rate of virus production (how parallel are the slopes?), peak titer (if titers plateau, are they plateauing at the same max titer?), etc.
edit: i know you're looking for just an answer, but the best way esp. if you want to publish your work is to have your own (supplementary) data you did yourself under your own laboratory conditions to test kinetics etc. unless the answer is accepted by literally every specialist in the influenza field.
Virus adaptation to a host is mostly receptor mediated, when change there is in virus or host receptor then species jump of virus will take place. One can not expect the same embryonic progenitor stem cells receptor in adult cells .
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