Is there any miRNA fluorescent labeling kit (or methods) that does not affect (add) the length of the miRNA?

I want to label my target miRNA and see the miRNA trafficking. But I don't want to use any plasmid or radioactive label. I need the fluorescent label that will not affect the length of my...

06 December 2020 8,471 3 View

Can too much of Tris-Cl buffer inhibit PCR?

I accidentally resuspended my stock primers (100 uM) in tris-Cl (pH 8.0) 1 M instead of 10 mM. I do PCR with 20 uM dilution from the stock. Will the high concentration of Tris-Cl inhibit PCR? I...

12 July 2020 2,971 10 View

Paired t-test or Independent samples t-test?

Hi, I am working on the home advantage. And examining the difference of a player's performance between playing at home and away. However, the sample size is different because the player played...

24 February 2020 7,053 3 View

Is it possible to wash PPFE slides that have been put through fluorescent reaction and stain them again?

I have some 3 year old PPFE slides of human brain that have been reacted with primary antibodies then reacted with Alexa Fluor secondary antibodies. The DAPI on the slides is still visible but the...

02 May 2019 7,390 2 View

What type of statistical tests on SPSS should I use for my study?

Hi I am carrying out a study investigating the association between age and password strategies among online users. I have collected my data, and inputted it on SPSS, however, I am not sure which...

10 December 2018 1,002 7 View

Can docking be used to predict a peptide's target receptor?

Let's say I have an array of receptors. Given a peptide, can protein-peptide docking be used in predicting the peptide's target among the receptors? If so, how? If not, why? Can you recommend me...

24 July 2018 3,569 4 View

Fc block not working for Flow Cytometry?

Good day all! I would greatly appreciate help with the following issue: Our lab is trying to characterize mouse lymphocytes for Tbet, EOMES, and PD-1. When I ran the samples the first time, the...

28 March 2018 1,225 3 View

In general speaking, how long can I keep antibody-labeled sections in wash buffer after the staining of secondary fluorescent antibody?

If the mounting is delayed for a day or two, would the specific binding being greatly reduced?

05 December 2017 4,103 3 View

Call for Papers: Australian Gender Economics Workshop (AGEW), Feb 2018, Perth Australia ?

15 October 2017 9,870 4 View

About EPMA data; (WDS only), for smectite.?

28 January 2017 3,785 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View