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Questions related from Vasishta Polisetty
My intention is to do an ELISA with brain homogenate coated on the base of a plate -> Adding a sample that might have antibody that binds to the homogenate -> Add a secondary and look for...
08 October 2024 5,563 3 View
I am planning to run flowcytometry on cell suspensions from brain tissue to look for cells that bind to certain neuronal surface antibodies. 1. Do existing dissociation methods work for adult...
26 October 2023 8,631 1 View
"Spontaneous coiling is the earliest motor behavior shown by the zebrafish embryo starting from 17hpf. The article attached says that the coiling appears at 17hpf and its frequency increases,...
20 June 2017 1,835 1 View
We do not know the etiology of many diseases. For example Crohn's disease. For that are doing a lot of risk factor analyses studying whether a particular factor is predictive or protective. My...
11 March 2017 9,667 3 View
Dermatophytes are cutaneous keratinophilic fungi. They cause concentric skin lesions. As a sample, usually skin scrapings are collected. When viewed under the KOH mount, they appear transparent...
23 November 2016 5,624 8 View
There are very few animals with polyploidy like salamanders. Why is it that polyploidy is so uncommon in animals? On the other hand, there are numerous examples of polyploid plants. If it is...
22 November 2016 6,379 4 View
According to Robbin's Pathology, hyperthyroidism leads to an overactivity of the sympathetic system. It also goes on to mention that this sympathetic hyperstimulation in the gut leads to increased...
22 November 2016 3,648 3 View
I know tat ImageJ has options to count cells either manually or automatically but my protein of interest lights up the synapses and hence can't use both. I thought of measuring the mean grayscale...
05 November 2016 4,867 2 View
In sexually transmitted infections, it is important to differentiate a painful ulcer from a painless one. This is because painless ulcers are seen in the chancre of primary syphilis while painful...
28 October 2016 8,703 9 View
The inside of the heart is covered by endothelium throughout. Why are valves predisposed to endocarditis? I know it can affect other structires like the septum, chordae etc. But why the valves?...
11 October 2016 5,280 2 View
We learn that the stroke volume is the same for the right and left side of the heart. When the stroke volumes are same, does it imply that the volume in the capillary bed and the systemic bed are...
09 October 2016 8,990 2 View
Since almost all the blood/serum parameters measured in diagnostics are concentrations, how valid are absolute values in case of hemoconcentration or hemodilution? For example a low platelet count...
24 August 2016 688 3 View
A neutralising antibody is one, which in an in-vitro neutralisation assay reduces the viral infection in its presence. This is because it hinders in the infection process. (correct me if wrong) On...
15 August 2016 4,612 8 View
Hemoglobin is formed by the tertiary structure of 2 alpha chains and 2 beta chains. The alpha is normal in sickle cell anemia, but the beta chains have a mutation and form Hb-S. But, in a sickle...
17 July 2016 8,454 8 View
Reverse cholesterol transport is the transport of cholesterol from the tissues back to liver/VLDL. My question is why do the tissues have this extra cholesterol in the first place (which they...
13 July 2016 3,213 6 View
We are doing a prevalence study on Obstructive sleep apnea (OSA). There is an established association with neck circumference. Neck circumference increases with height and there is a variable...
06 July 2016 9,290 3 View
In parasitology, we do a flotation test in a solution of zinc sulfate to easily classify the eggs for identification. It is known that eggs of Ascaris float only if fertilised. What change(s) does...
03 July 2016 5,124 4 View
Is diarrhoea just collateral damage to the host or has it got an evolutionary advantage to it? I'm not asking w.r.t a specific species because many microbes share a similar structure of the HLT of...
03 July 2016 8,066 4 View
Sucrose acts as a cryoprotectant by removing the water (by exosmosis?) Does this alter the size of the cells? Technically the cells should shrink due to the 30% sucrose hypertonic solution being...
28 June 2016 4,410 3 View
Most protocols suggest the use of DTT (Dithiothreitol), a reducing agent in the isolation QB buffer for extraction of protein from plant tissue. I plan to run an SDS-PAGE gel. Is there an...
21 June 2016 787 4 View
I am to isolate the retina and run a SDS-PAGE western blot. Is it better to store the isolated tissue directly at -80C or to extract the protein immediately and store the extract at -80? Duration...
14 June 2016 4,875 4 View
