I like to use e.coli cells (pellet) in buffer, which should convert a aromatic and low water soluble molecule. Could DMSO help to overcome the transport limitation ?
Or have someone expericence with digitonin e.g. ? http://en.wikipedia.org/wiki/Digitonin
Though i have experience with plant based whole cell biotransformations, DMSO to some extent (< LD 50 dosage) might help to increase membrane permeability. Also cyclodextrins are another option open for Plant based whole cell biotransformations to significantly improve substrate accessibility to the intracellular enzymatic machinery
As biotransformation is done in aqueous system, therefore, the substrate should be soluble in water-miscible solvents e.g. acetone, ethanol, methanol, etc. DMSO or DMF are our last choices. Hence, we can say that it is good "if substrate is insoluble in other organic solvents".
Yes, DMSO enhances the permeability of membrane.
DMSO will enhance the solubility, but probably will not affect the transport. Depending if it is a resting or growing cell system, and what type of conversion you envision, you could try to add permeabilization agents (if this is the major problem). Growing E. coli cells can tolerate some DMSO (>5%). On the other hand, DMSO frequently renders DSP difficult...
- Badges
- Science topic