I am trying to clone pre-miRNA in pcDNA3.1+ vector.
Wanted to know does directionality is important for this cloning ? or my insert in any orientation of promoter will be ok.
Thank you
Regards,
Pawan
For analyzing the expression of miRNAs, the three approaches are commonly used 1) forward genetics, 2) directional cloning and 3) bioinformatic analyses.
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