Does directionality matter while cloning Pre-miRNA in expression vector?

09 September 2015 1 3K Report

I am trying to clone pre-miRNA in pcDNA3.1+ vector.

Wanted to know does directionality is important for this cloning ? or my insert in any orientation of promoter will be ok.

Thank you

Regards,

Pawan

Ishtiaq Qadri

For analyzing the expression of miRNAs, the three approaches are commonly used 1) forward genetics, 2) directional cloning and 3) bioinformatic analyses.

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