Pawan Upadhyay

3 Questions 14 Answers 0 Followers

Questions related from Pawan Upadhyay

Does directionality matter while cloning Pre-miRNA in expression vector?

I am trying to clone pre-miRNA in pcDNA3.1+ vector. Wanted to know does directionality is important for this cloning ? or my insert in any orientation of promoter will be ok. Thank you Regards, Pawan

09 September 2015 2,958 1 View

Which software is used to count softagar colonies from images?

I have done softagar and taken 10 images from each wells. So, in total 60 images per 6 well plates. It seems to be tedious job to count these colonies in these images as I have multiple...

05 May 2013 1,703 4 View

What may be "oncogenic addiction" part in cancer cell?

I want to know ,which protein in cancer cell makes them addicted for that. Without which they can not survive (oncogenic addiction),just like other addictions for humans.

10 October 2011 278 8 View