What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

(Scale request) Anyone has the Malay version of Eating Attitude Test-26/40 (EAT-26/40)?

Dear Researchers, My research team is conducting a eating disorder related research in Malaysia and we would like to use the Malay translated version of EAT26/40. I have tried to email the...

24 January 2021 8,925 2 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

What corporate social responsibilities do social media companies have in relation to the use of psychographic profiling in advertising ?

What are the responsibilities of CSR ? What social media companies have done to protect the privacy or information of their users ?

12 October 2020 433 5 View

May I know is Triple Negative cell line MDA-MB-468 is auto fluorescence ?

I am doing immunofluorescence using MDA-MB-468 cell to determine ICAM-1 and E-cadherin with Santa Cruz Biotechnology ICAM-1 (G-5) mouse anti-human (Primary antibody), Santa Cruz Biotechnology...

27 August 2020 1,641 4 View

Can anti-Fas (anti-CD95) be used to prepare apoptotic cancer cell as antigens for DC to present antigen?

Researchers usually used irradiation (such as x-ray) to induce cancer cell apoptosis to prepare available antigens for DC to uptake that present the antigen to T cells. Can other apoptotic methods...

26 August 2020 5,445 4 View

How to generat a ASCII format data in GIS?

Dear you all: Recently i met with such a problem: i tryed to generate a ascii format buffer in gis, but i failed because it's headers are different from the research data. Failed result are shown...

11 August 2020 5,040 3 View

Removal of dead cells before harvesting for Proteomics?

Dear all, I am culturing some suspension cell lines. I am planning to perform proteomics (LC-MS/MS) experiments on them. Unlike adherent cells (viable cells are adhering to the substrate and...

03 March 2020 2,329 3 View

Enrichment of AHA-tagged proteins: Should I use DBCO- or Alkyne-based methods?

Dear colleagues, I would like to seek your kind advice on click chemistry enrichment of azidohomoalanine (AGA)-tagged proteins. From literature, I learned that there are 2 main avenues, either...

18 February 2020 8,725 2 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View