Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

How can i send the Mandelys reference Library file ???

hi to all my dear colleagues, i need your help concerning finding the proper way to send and prepare the library file of references by Mandely??

06 July 2020 383 5 View

I would like to quantify cytokines from mice brain using ELISA, What is the total protein concentration i suppose to add in each well?

Hi everyone I want ant to use ELISA to quantify tnf a ,il-1b and inf g in Half brain of mice. I was wondering if any one has done this exactly before and can tell me which concentration of...

07 May 2019 7,701 3 View

What does it mean by negative values for number of genotypes identified by a primer in detecting the polymorphism ?

So i am recording the polymorphic statistics of iPBS primers and one of the parameters estimated is R (resolving power). From an equation (0,15x + 1,78 = R) i can detect the number of genotypes...

14 February 2019 9,111 1 View

How can I store stool specimen for Molecular diagnosis for human parasites Is there a difference of storing for PCR or real PCR or multiplex ?

Molecular stool

08 January 2019 8,186 3 View

No title

how prepare this mix morality in constant total volume 800 ul

04 June 2018 9,451 3 View

What is the different between UPGMA and Neighbor-Joining Methods in the building of the phylogenetic tree?

the methods of building the phylogenetic tree

12 April 2017 1,050 2 View

Can anyone provide me with some papers regarding the nutrient deficiency within the soil in Iran ?

29 January 2017 650 4 View

Vitamin B12 detection & determination?

What is the best method to detection and determine of Vit. B12 from bacteria?

10 November 2016 6,035 3 View

How can you verify if your research work is novel or not?

03 March 2016 9,154 33 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View