Does anyone who has experience in cultivating 3D spheroids of HepG2's using the hanging drop plates know the protein content of 12 day spheroid?

10 October 2013 1 450 Report

I am wanting to perform 2D gel eletrophoresis on treated 3D spheroids grown using a hanging drop plate and need to find out how much protein would be available in 12 day old spheroid cultures.

Vijay Kumar

The following publications on HepG2-based spheroids could be of immense help:

1. K. Wrzesinski et al. (2013). "HepG2/C3A 3D spheroids exhibit stable physiological functionality for at least 24 days after recovering from trypsinisation.

Toxicol. Res. 2, 163-172.

2. http://www.insphero.com/images/documents/InSphero-HepG2-microtissues.pdf

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