Does anyone have or know about a protocol for isolating microvessels from mouse brain to study tight junction proteins?

There are different protocols for microvessel isolation from mice or rat. Which protocol should I follow to isolate microvessels from mouse brain so that I can evaluate different tight junction...

05 January 2021 696 2 View

How can I use an hybridization of ontology and machine learning for text summarization ?

my work consists of creating a system for text summarization in a specific domain(crisis management) using a hybridization of ontology and machine learning algorithme ,my question is how to use...

23 February 2020 8,327 2 View

Gauge Pressure and Operating pressure on combustion system?

Hi all, Please I have a question about the pressure: I am working on combustion, and I want to study the effect of pressure in my geometry, I couldn't understand where sould I put the pressure for...

14 February 2020 7,977 3 View

What will happen if I add the NO models (NO thermal and NO prompt) for the flamelet model using GRI mech? How Fluent works in this case?

Hi everyone, The GRI mechanism contain all the reactions, and species of NOx, and in the Fluent we can also find the NO models ( prompt, thermal and N2O..), when I added the models I found a big...

22 December 2019 3,785 5 View

Is there a difference in binding site when comparing anti-CD279 (PD-1) clones EH12.2H7 and EH12.1?

Hi all, I would like to know if these clones of anti-PD-1 antibodies (EH12.2H7 and EH12.1) share the exact binding site. Thank you in advance for your help, Sabrina

19 March 2019 4,908 3 View

Is the title and goal of my project suitable?

I am starting works on a project with current working title: Gender Roles in African Philosophy: Appraising the place of women in African traditional society. A Case Study on Setswana and...

20 January 2019 2,161 4 View

How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions?

How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions. Please try to Repair Overlapping Contact Regions.

01 December 2018 7,320 3 View

What to use for correlation between ordinal scales?

Hello, I have a variable "firm's results" which is an ordinal scale and 5 variables for direct marketing, each of them in ordinal scale too. I know that I can use Pearson if I want to know the...

05 November 2018 1,535 3 View

I have tilt in my geometry, so Is there any way for doing Line moving with the flow ?

Dear professors and research scholars, I have a query for the code Fluent My geometry is not symmetric, I have tilt in my geometry so Is there any way for doing Line moving with the flow !!!...

19 February 2018 8,538 4 View

I have a problem with my results from FLUENT when I imported in ORIGIN, I have other lines! Does that mean the problem with the mesh or secondary air?

Dear professors and research scholars, Attached is a figure showing the distribution of the temperature along the central axis of combustor Fuel, I have problems with my results, When I put it in...

09 February 2018 738 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

08 February 2021 4,557 2 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

What should my objective ?

I am going to start my master program (msc agri hons) research in rull of potassium in tomato vigetable crop so what should my objective please guide me Thanks

13 January 2021 4,635 6 View

What is this culture medium for?

Here is the composition of a culture medium named BM medium. It doesn't contain carbon source and nitrogen source, while after cultivation something grow on that. Could anyone explain what...

31 December 2020 8,060 5 View

Does anyone recycle cell culture media and if there is a person what is the procedure and WHY do they do that?

our team researching to cultivated meat, and there is some question related to cell culture media and recycling cell culture media: 1) is cell-cultured media recyclable? 2) has anyone recycled...

27 December 2020 1,467 4 View

How to empirically approach Responsible leadership?

05 November 2020 8,021 5 View

Tomato pollen internal membrane dye?

Hello everyone, I wonder if you can help to suggest any internal membrane dye that would enable me to stain the generative cell in tomato? unfortunately I have no marker tagged to the cell but I...

28 October 2020 2,335 3 View