Salam , there are more than one way to extracted DNA .but if you insist to use Prepman Ultra sample reagent , I guess this file will help you .

There is a protocol........

https://www.thermofisher.com/order/catalog/product/4318930

Hello Dr.Alejandra

You can find the useful references on attachment 1

https://www.thermofisher.com/order/catalog/product/4318930

2

https://assets.thermofisher.com/TFS-Assets/LSG/manuals/cms_042136.pdf

3

https://docs.appliedbiosystems.com/pebiodocs/04318924.pdf

4

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=0ahUKEwj1ze_d-crWAhVGOMAKHWyvD58QFgg4MAQ&url=https%3A%2F%2Fvetmed.tamu.edu%2Ffiles%2Fvetmed%2Fdna-core-lab%2Fmicroorganism%2520identification%2FPrepManUltraQuickRef_000.pdf&usg=AFQjCNFQn1ja46uDS2L90ZvpEfSGsM3Yfg

5

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&cad=rja&uact=8&ved=0ahUKEwj1ze_d-crWAhVGOMAKHWyvD58QFgg6MAU&url=https%3A%2F%2Fwww.bioz.com%2Fresult%2Fprepman%2520ultra%2520sample%2520preparation%2520reagent%2Fproduct%2FApplied%2520Biosystems%2520Inc&usg=AFQjCNFRprVWglydnnngLHK1Akylhc9kAQ

6

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&cad=rja&uact=8&ved=0ahUKEwj1ze_d-crWAhVGOMAKHWyvD58QFgg_MAY&url=https%3A%2F%2Fwww.bioz.com%2Fresult%2Fprepman%2520ultra%2520sample%2520preparation%2520reagent%2Fproduct%2FLife%2520Technologies&usg=AFQjCNH3ea_uBBVjBWpbH9R9-uPuhrOwzg

7

https://onlinelibrary.wiley.com/doi/10.1046/j.1472-765X.2002.01231.x/full

8

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0ahUKEwj1ze_d-crWAhVGOMAKHWyvD58QFghMMAg&url=http%3A%2F%2Finfectmet.lgcgroup.com%2Fwp-content%2Fuploads%2F2013%2F02%2FHLT08-INFECT-MET-D1.2.1.pdf&usg=AFQjCNF8RMH3ZCZJrx4lVgbmxur52CHKeQ

9

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&cad=rja&uact=8&ved=0ahUKEwj1ze_d-crWAhVGOMAKHWyvD58QFghRMAk&url=https%3A%2F%2Fquizlet.com%2F152488763%2Fchapter-4-genomics-flash-cards%2F&usg=AFQjCNFxxx2319hZKYvzTKXV5PJ6cYbA5Q

10

https://aem.asm.org/content/74/8/2492.full

11

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=12&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCC4wAQ&url=https%3A%2F%2Fwww.yumpu.com%2Fen%2Fdocument%2Fview%2F19384142%2Fprepman-ultra-dna-extraction-protocol&usg=AFQjCNHTS1faU1dc0ZWAcYWYvTmtrpYabQ

12

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCDMwAg&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4944934%2F&usg=AFQjCNEsn9Jv-K0TZG9Tto_9Ky5qy4v-EQ

13

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=12&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCC4wAQ&url=https%3A%2F%2Fwww.yumpu.com%2Fen%2Fdocument%2Fview%2F19384142%2Fprepman-ultra-dna-extraction-protocol&usg=AFQjCNHTS1faU1dc0ZWAcYWYvTmtrpYabQ

14

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCDMwAg&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4944934%2F&usg=AFQjCNEsn9Jv-K0TZG9Tto_9Ky5qy4v-EQ

15

https://jidc.org/index.php/journal/article/download/25771468/1269/

16

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=15&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCD8wBA&url=http%3A%2F%2Fthescholarship.ecu.edu%2Fbitstream%2Fhandle%2F10342%2F4023%2FKosch_ecu_0600D_10593.pdf%3Fsequence%3D1&usg=AFQjCNHy8XJ9wYUjfNGucaRW28MvFt1vRg

17

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=16&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCEcwBQ&url=http%3A%2F%2Fdocplayer.net%2F10329795-Fast-microseq-d2-lsu-rdna-fungal-identification-kit.html&usg=AFQjCNHGawzxO_gGOy3RHihCts_Om3vPIg

18

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=17&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCEkwBg&url=https%3A%2F%2Facademic.oup.com%2Fmmy%2Farticle%2F50%2F6%2F619%2F977069%2FMultilocus-sequence-typing-of-serial-Candida&usg=AFQjCNFYBscVES9JQLIeozOZzpaW9PNYeg

19

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=18&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCEswBw&url=https%3A%2F%2Fbmcmicrobiol.biomedcentral.com%2Farticles%2F10.1186%2Fs12866-014-0326-z&usg=AFQjCNHl8lM_ADoLSQPryVfFQMBIWoOguw

20

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=19&cad=rja&uact=8&ved=0ahUKEwi2roCr-8rWAhVjFMAKHc-9Ab44ChAWCFEwCA&url=http%3A%2F%2Fwww.academia.edu%2F33504963%2FIdentification_of_bovine_material_in_porcine_spray-dried_blood_derivatives_using_the_Polymerase_Chain_Reaction_technique&usg=AFQjCNGF33LBtfh1ifhueFqE33Vw_UVKyg