Minghui Liu

4 Questions 2 Answers 0 Followers

Questions related from Minghui Liu

How to optimize my protein crystallization conditions?

I'm trying to get a protein crystal with a new compounds and there was reported x-ray structure of this protein. However, when I follow the crystallization condition of related paper, I get very...

17 June 2021 7,438 8 View

Does anyone use microplate reader to detech GFP fluorescence in 96well plate?

I transfected Hela cells with GFP-tagged protein in corning-3603 black clear bottom 96-well plate and tried to use biotek cy3 microplate reader to detect GFP flurorescence intensity with...

17 September 2020 9,841 7 View

Does anyone use normal clear 96wells plate to test cell viability by using CellTiter-Glo?

In our lab, we use Corning 3903 White 96 wells plate to culture cells and use Celltiter-Glo (Promega G7570) to test cell viability, I wonder if we could use normal clear 96 wells plate, such as...

20 July 2020 8,200 3 View

Can I store my BL21-DE3 plate after transformation for a few days at 4℃?

I did a plasmid transformation using BL21-DE3 bacteria yesterday for protein expression, however I couldn't grow them in LB, so I wonder if I can store the BL21-DE3 at 4℃ for one day, or I need to...

16 July 2020 2,381 4 View