Minghui Liu

6 Questions 2 Answers 0 Followers

Questions related from Minghui Liu

How to get high-purity murine KO cells?

Hi, I'm trying to knockout one gene in murine PDAC cell lines. However, I found the knockout efficiency is only about 70% (by gDNA sequencing analysis). Because it's not a good idea to pick single...

06 April 2024 6,193 1 View

How to optimize my protein crystallization conditions?

I'm trying to get a protein crystal with a new compounds and there was reported x-ray structure of this protein. However, when I follow the crystallization condition of related paper, I get very...

17 June 2021 7,480 8 View

How to purify my proteins which get precipitating in low salt buffer using HiTrap SP HP column?

I express and purify my protein by Ni-NTA beads in 50mM Tris 8.0 and 150mM NaCl and get enough protein. But when I want to use ion exchange column to purify it, proteins get precipitating even...

05 June 2021 8,806 0 View

Does anyone use microplate reader to detech GFP fluorescence in 96well plate?

I transfected Hela cells with GFP-tagged protein in corning-3603 black clear bottom 96-well plate and tried to use biotek cy3 microplate reader to detect GFP flurorescence intensity with...

17 September 2020 9,925 7 View

Does anyone use normal clear 96wells plate to test cell viability by using CellTiter-Glo?

In our lab, we use Corning 3903 White 96 wells plate to culture cells and use Celltiter-Glo (Promega G7570) to test cell viability, I wonder if we could use normal clear 96 wells plate, such as...

20 July 2020 8,341 3 View

Can I store my BL21-DE3 plate after transformation for a few days at 4℃?

I did a plasmid transformation using BL21-DE3 bacteria yesterday for protein expression, however I couldn't grow them in LB, so I wonder if I can store the BL21-DE3 at 4℃ for one day, or I need to...

16 July 2020 2,457 4 View