Meagan Doppegieter

4 Questions 2 Answers 0 Followers

Questions related from Meagan Doppegieter

What could improve my small nerve fiber staining?

I have spent several months to optimize our nerve staining in human skin biopsies. We fix our biopsy 24h in Zamboni at 4 °C followed by 24h submersion by parafilm in 30% Sucrose at 4°C. Then they...

02 February 2022 8,877 1 View

What determines the concentration of FBS/FCS in cell culture media?

I have been reading a variety in FBS concentrations in cell media ranging from 8% to 15%. Does anyone has factual reasons as to why a certain cell type needs more FBS? Does it really matter or is...

05 March 2020 4,040 3 View

How to determine nerve functionality (in a skin biopsy)?

I am struggling to find a quantitative method for nerve functionality in skin biopsies. Would it be possible to quantify neuropeptides/ACh as a direct relation to nerve functionality (taking into...

24 February 2020 6,524 0 View

In what way is Botox degraded in the body, is it metabolized or do nerves simply regrow?

Treatments with botox have variable lenghts of effectiveness. However, between a period of 3 to 8 months, the botox is not effective. What is the underlying mechanism in the decrease of inhibition...

16 January 2020 9,654 3 View