How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Which indexation is better Scopus or Web of Science?

Both are very repetitive indexing agencies.

26 February 2021 9,455 4 View

Anybody write me the name of scopus journals in commerce, management, economics areas. Which publish without any charge?

Please write those scopus journal names for which you have good experience with them.

24 February 2021 6,210 1 View

How to extract water soluble amino compound from after aqueous work up?

4-Dimethyl amino pyridine is used in reaction.Product formed contains amino group along with amide & is highly water soluble. To remove 4-DMAP aqueous work up done. Please give solution. Thanks.

21 February 2021 1,883 1 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

How to decrease the time for publication of paper in a journal ?

It takes a lot of time in publishing the paper in the journal, from few months to a year so how one can able to decrease the time for publication.

17 February 2021 2,997 14 View

Can anyone suggest a software for running a plant tissue culture lab?

A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.

17 February 2021 3,015 5 View

Do you think NCC and 3 year service at army base must be compulsory to make the students loyal and discipline to the nation?

Decipline and loyalty for nation is the need of today.

09 February 2021 6,928 3 View

Is the oncogene transcription level stable between different passages in hUC MSC ?

23 July 2019 4,243 3 View

How to predict the structure of a protein from a DNA sequence?

I have a sequence of DNA for an oncogene with a novel oncogenic mutation. I want to know how does this mutation affect the structure of the protein produced by that gene. What softwares can help...

23 July 2019 9,194 1 View

Will miRNA transfection affect cell morphology?

Hi Scientist, I am transfecting an oncogenic miRNA into a breast cancer cell line and based on your experience in the mammalian cell line, will such transfection affect the cell morphology?

06 July 2019 7,378 8 View

Is the oncogene transcription level stable between different passages in hUC MSC ?

06 July 2019 9,561 3 View

How to choose cell line for research?

03 April 2019 6,434 2 View

Melting temperature (Tm) of G-quadruplex DNA?

I am using G-rich oligonucleotides to form corresponding G-quadruplex DNA using a standard protocol. Specifically, I am using 22mer HTelo and 22mer human oncogene promoter with Tm of 57.7 and 61.8...

09 October 2018 9,328 9 View

Is Target Capture NGS Assay Suitable for Differential Expressed Genes Analysis ?

Dear RG community, I have a dataset composed by 100 samples of Acute Lymphoblastic Leukaemia Patients sequenced by Illumina Tru-Sight RNA PanCancer - Target Capture Next Generation Sequencing (...

09 October 2018 204 1 View

Melting temperature (Tm) of G-quadruplex DNA?

I am using G-rich oligonucleotides to form corresponding G-quadruplex DNA using a standard protocol. Specifically, I am using 22mer HTelo and 22mer human oncogene promoter with Tm of 57.7 and 61.8...

07 October 2018 3,750 0 View

Is CREBBP a tumor suppressor or a tumor promoter?

There is plenty of literature evidence that state that CREBBP is a tumor promoter and also the contrary. Can it's functions be generalized as an Oncogene/ Suppressor? Since it impacts both kinds...

04 September 2018 619 3 View

How could I stain extrachromosomal DNA by fluorescence in situ hybridization (FISH) ?

Hi everyone, Is there anyone who has experience on staining extrachromosomal DNA by fluorescence in situ hybridization (FISH) ? I would like to stain extra oncogenes on extrachromosomal DNA and...

07 August 2018 9,739 4 View