Does anyone know one antibody that works against Mycobacterium spp?

Anti-Mycobacterium antibody: does anyone know one antibody that works against Mycobacterium spp. (mainly for immunofluorescence)? Many thanks in advance! Cristiane

04 January 2021 9,724 3 View

Can likert-type statements formulation derive solely from literature review?

28 October 2020 9,951 3 View

How to report analytical uncertainty calculations in a research paper about environmental contamination?

We submitted a paper investigating trace elements levels in multiple abiotic and biotic matrices using analytical protocols already established. One of the reviewers required us to report...

27 October 2020 8,453 4 View

Does 2-methoxyethanol reacts with air?

Hello, I'm trying to use 2-methoxyethanol/Cellosolve to dilute methylumbelliferyl phosphate for phosphatase activity analysis. However, we never used this solvent before. It came with a sure/safe...

27 October 2020 7,302 1 View

Can we predict differential expression genes (MicroRNAs) by analyzing a DNA Methylation dataset?

29 July 2020 7,333 2 View

How can I design an experiment capable of demonstrating if my cells can grow without a substrate?

Dear Researchers, I am incredibly confused about the in vitro colony forming assay. I was asked to perform such assay to assess my cell models capability to grow without attachment to a solid...

10 June 2020 6,230 3 View

How do I convert a list of smiles to Name in MOE?

I have a MOE database with molecule codes and smiles that I have to convert to name. What is the easiest way to do this? Thank you.

04 May 2020 6,987 3 View

What is the best protocol for evaluate TMR-peptide?

I apologize if my question seems to naive, but I would like to do a protocol to determine bio-distribution of a peptide coupled to TMR in an animal model however, I am having some difficulties in...

07 July 2019 5,664 2 View

Anyone could give some tips for fish brain microdisection?

I want to isolate fish brain specific zones like telencephalon, cerebelum, opticum tectum. I am encoutering with the problem of hardness of tissue because in the moment we try to take fish brain...

30 April 2019 8,841 1 View

In which countries of the world have digital textbooks been used as a substitute for printed models?

I think the digital textbook is an important additional resource to the printed book for enabling portability and choices for the student.

21 March 2019 9,022 6 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View