Natália Ferreira Mendes

4 Questions 5 Answers 0 Followers

Questions related from Natália Ferreira Mendes

Which is a good alternative for ethidium bromide solution in genotyping?

I have used ethidium bromide solution for genotyping different mouse strains successfully, but the laboratory that I am working on currently does not allow it. Currently, I have been using a...

16 September 2021 6,838 3 View

Does anyone perform RNA-seq from samples with low levels of RNA (5-10 pg/ul)?

I am wondering if these low levels of total RNA the samples are enough for RNA-seq. Does anyone already did it or has any suggestions to get a reliable data for bioinformatic analysis?

19 August 2021 3,880 7 View

Which is the best antibody to label GFAP (mouse or goat) on IHC-fluorescence (brand and catalog number)? And which secondary you conjugate?

I need to get good images of astrocytes in the hypothalamus, but the antibodies I have been using are not labeled very well, while the DAPI and my protein of interest appear perfectly. What...

14 January 2017 5,868 3 View

To study reward system, what is the best antibody to show dopaminergic neurons in immunohistochemistry?

Studies show two types: dopamine-beta-hydroxylase and tyrosine hidroxylase.

03 June 2014 3,849 2 View