I wish to isolate SP-D proteins and I was not sure why the protocol mentions the use of MnCl2 as an elution buffer to elute the SP-D protein from the maltose agarose beads column. Any help would be appreciated! Thanks alot!
According to this paper, maltose binding by SP-D was inhibited by manganese and other ions.
Article Surfactant protein D (SP-D) is a divalent cation dependent c...
Thankyou very much!
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