Does anyone know why 50mM of MnCl2 is used as an elution buffer during Surfactant protein D isolation from Maltose agarose bead columns?

I wish to isolate SP-D proteins and I was not sure why the protocol mentions the use of MnCl2 as an elution buffer to elute the SP-D protein from the maltose agarose beads column. Any help would...

26 June 2023 5,598 2 View