What test should I use?

15 February 2021 911 5 View

How remove endotoxins from DEAE-Sepharose?

Hi to everyone! I have to validate endotoxins removal from DEAE-Sepharose I use for the purification of my hormone. The resin undergoes to a sanitization protocol with NaOH. To demonstrate the...

29 October 2020 9,141 4 View

How can I roughly estimate the current denisty in an FPGA?

Hello, I am currently working on the estimation of the electromigration of an FPGA. We just performed the construction analysis so we know all the values of wire width and height for each metal...

22 June 2020 2,743 3 View

What type of proteasome inhibitors is better to use in tissue lysate?

Hi all. I am looking for a proteasoma inhibitor to use in my tissue lysate. I found MG132; lactacystin and PR39. Anyone know which of these is more effective to use in a tissue lysate?, Do I need...

16 April 2020 4,088 3 View

What is the (average) DNA yield (ng) from one single nematode?

Do you know where I can found published information about DNA quantity (in ng) from one single nematode? Any publications/books to suggest me? I Know it seems a simple and basic information, but...

31 March 2020 6,710 1 View

Can I use Arrhenius Relationship to determine Accelerated factor , when test temperature is lower than use temperature?

Hello, I have a question regarding the acceleration factor. We know that the lifetime (TF) of a component can be expressed as: (1) TF=A0exp(Ea/(kT)) where A0 is a constant (component...

01 March 2020 6,635 6 View

Does anyone have experience with fMLP as a neutrophil chemoattractant in a transmigration assay?

I am planning to perform a transmigration assay (transwell plate) in which are involved neutrophils , therefore I will use fMLP as a chemoattractant. I did find some papers that describe "The...

19 February 2020 5,855 3 View

How much does it cost a Frantz Magnetic separator?

I would like to know the price of a Magnetic sperator to manage the separation of zircons, apatites and heavy minerals. I'm not interested in explanation of other methodologies, i just would like...

09 January 2020 4,590 2 View

Could you help me to find a paper?

16 August 2019 3,725 3 View

Where to publish a short descriptive note about a novel behaviour?

I have found a novel behaviour in an insect and I would like to publish it, but I do not know where to publish something that is just descriptive (with photos and videos) and does not includes any...

13 June 2019 401 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View