Best analysis for this longitudinal study?

Hi everyone, I need help for what analysis is better for this longitudinal study. In my study, I measured the attachment security of children at 5 time points. My dubts are that: 1) spacing...

07 June 2020 9,834 2 View

Cardiac rupture following electrocution in birds: is it common?

24 November 2019 9,676 2 View

Could you suggest a microplate reader for bacterial kinetics?

I would like to purchase a microplate reader to assay bacterial growth kinetics when exposed to different conditions (i.e.:salt concentration, different sugars, etc.). Ideally, I am looking for an...

11 September 2019 3,129 2 View

Can anyone share a good protocol for coating plates/dishes/coverslips for mouse hematopoietic stem cells?

I am currently facing problems with my cell attachment. Can anyone help me suggesting a protocol for coating plates/dishes/coverslips with Poly-L-Lysine for hematopoietic stem cells? Would you...

12 November 2018 6,693 6 View

Did someone use an antibody against caspase-9 for immunoprecipitation from mouse cell lysates?

14 December 2015 9,546 2 View

Can anyone suggest some good laboratories in Northern Italy for standard environmental testing?

I need to identify some good Labs placed in Northern Italy to be able to make vibrations, thermal cycling, ingress protection, salt spray and cold soak tests for automotive application (and not...

11 November 2015 5,518 5 View

Who are the worldwide experts/opinion leaders in primary cancer cells 3D culture?

25 October 2015 1,063 3 View

Does Someone know if FITC is soluble in chloroform?

I know it is soluble in ethanol, acetone, DMSO, DMF; someone knows about chloroform?

19 March 2015 4,740 2 View

Why don't my EBV transformed B cells secrete antibody?

Dear all, I transformed with EBV B cells from total PBMC and after 1 month I isolated IgG+ cells using Miltenyi beads. Keeping in culture these cells with CpG and without, I got proliferation but...

16 March 2015 9,839 5 View

Fluorescein stabilization in ethanol solution for temperature measurements

I'm working since several months with a solution of Fluorescein Disodium (C20H10Na2O5) and Ethanol. So far, the spectrum of this solution is changing due to unknown parameters and I would like to...

01 January 1970 7,629 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View