I am looking to make a start on a metabolomics approach to cell characterisation over time in culture. One approach I am thinking of is the use of metabolomics.
In summary, I want assess 5-6 key metabolites in CHO cells over a particular time frame. I have good knowledge, and experience, of how to conduct the experiment and obtain the data, using both culture media, and cell extracts. But I need help on ways to analyse this data as well as determine if any existing data is out there already.
I have found a number of papers that have conducted the same approach on CHO cells, and have provided very good summaries of data. But I was just thinking, is the raw data freely available? Where is this data located? And what programs are currently in use to analyse this data to identify trends or most abundant metabolites, etc.?
Your help is greatly appreciated, and I look forward to any answers to come.