What are the SPSS commands to calculate item discrimination index and difficulty index as part of item analysis?

Hi everyone! I am trying to find the "how to" on item difficulty and discrimination on SPSS and its interpretation. I have read about and performed the command analyze> scale> reliability...

21 September 2020 4,040 3 View

Does somebody know about an index that measures Populism among countries?

I'm looking for something similar to the Economic Freedom of the World Index but measuring Populism... I do not know if it exists. Thank you

22 June 2020 4,460 8 View

Does anyone have a protocol to do nuclear extraction for enzymatic activity assay?

I would like to measure enzymatic activity of some nuclear proteins, but I have troubles with the extraction and keeping its native form. Sonication seems to be a must in my cells to release...

23 September 2019 7,472 7 View

How to estimate and plot marginal effects form an interaction model with data panel in Eviews 10.0...?

Who knows how can I estimate and graph the marginal effects from an interaction model -regression data panel- in EViews10.0. Y = X + Z + XZ I need to graph the marginal effect of X on Y...

07 April 2019 2,013 1 View

How to use molecular sieves to dry Methanol or Ethanol?

So i'm trying to use molecular sieves to dry methanol, but i can't find a good tutorial on how to do it, any help would be greatly appreciated.

10 February 2019 299 3 View

How I quantify DNA in GENESYS 10S UV-Visible and Visible spectrophotomete?

Hi, I want to quantify DNA, I have a GENESYS 10S UV-Visible and Visible spectrophotometer, but I do not have the instructions. Could someone tell me what to do? Do I need some software? Thank you

10 August 2018 6,181 2 View

Can anyone give me a working protocol to extract DNA from Salmonella colonies using chloroform-isoamylalcohol (24:1)?

I need a working protocol to extract DNA from Salmonella colonies.

07 June 2018 7,384 3 View

Which has been the major contribution of the mechanical engineering to the development of renewable energies?

Contributions of the mechanical energy to the renewable energies (Utilization, discovery, creation, etc)

08 April 2018 8,984 5 View

Whats the recommended volume for 6-well plates in cell culture?

Hi, I am new to primary cell culture and I'll be working with chicken-derived dendritic cells. I'll be using GM-CSF and IL-4 to induce their maturation in 6-well plates with RPMI-1640 media. I...

05 February 2018 3,940 4 View

In Cancer Epidemiology, which indicator should be more suitable as estimator of burden of disease?

Dear RG community: I am currently working on an ecological study in order to analyze disease burden from cancer in my country. I am looking for the most suitable indicator of disease burden....

23 January 2018 8,059 11 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View