Hi all,
I`m frequently observing this peak at the very beginning of the elution of my nanoLC-MS/MS samples (mainly phosphoproteomics). It`s pretty abundant and elutes on a much wider RT range than the peptides in the sample. However, it seems to form 'sub-spikes' on that LC-peak.
Any ideas what that could be?
Thank you!
Have a look at the MaConDa database. Note they use exact mass. I can’t see anything that would correspond to your contaminant, but double check the mass and scan the database to get an idea of what it might be. It at least should give you a place to start.
The Zhang paper has a spectrum with a prominent 524 ion (charge stage not specified) and it’s not mentioned in the text. Nevertheless, you should check it out.
X. K. Zhang, R. C. Dutky, and H. M. Fales. Rubber stoppers as sources of contaminants in electrospray analysis of peptides and proteins. Anal.Chem. 68 (18):3288-3289, 1996.
The Keller list is extensive (but no 524 ion).
B. O. Keller, J. Sui, A. B. Young, and R. M. Whittal. Interferences and contaminants encountered in modern mass spectrometry. Anal.Chim.Acta 627 (1):71-81, 2008.
http://www.maconda.bham.ac.uk/browse.php
Is the contaminant also present when no sample is loaded (eg only solvent)?
Hi guys,
thanks a lot for the answers.
Great question, Simon. It does indeed seem to be sample specific, i.e. not in the blanks.
Okay, then I guess the next step is to check if it happens with all peptide digests or only to specific ones. Maybe you can narrow it down that way!
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biomolecules
- Peptides