Michael Plank

6 Questions 15 Answers 0 Followers

Questions related from Michael Plank

What should be distance between promoter and initiator-ATG?

Hi, I was wondering if anyone here has some insight into what should be the distance between the promoter and the translation start site / initiator-ATG when making a DNA construct? In...

03 October 2021 8,314 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

02 March 2021 502 1 View

Do you have recommendations for plasmids for yeast protein tagging?

Hi, would anyone have recommendations for a set of plasmids for endogenous C-terminal protein tagging in S. cerevisiae (i.e. plasmids as template for the PCR product to transform). I`m looking for...

19 November 2020 3,983 1 View

How to perform on-filter on-bead digestion?

Hi all, I`m trying to analyse PTMs (mainly phosphorylation) of a yeast protein by MS after enrichment of the tagged (e.g. TAP-tag) protein. I would like to perform the tryptic digestion on the...

02 October 2018 9,674 6 View

Protocol/settings for yeast proliferation assay in 96-well plates?

Hi, would anyone have a protocol or be willing to share their settings for a yeast (S. cerevisiae) proliferation assay in 96-well plates? We have a Tecan Spark 10M platereader, but I`d imagine...

20 June 2018 2,830 10 View

How can I use information from different protease digests for PTM localization?

Hi, it should be possible to enhance your confidence in PTM site localization by combining information from digests with different proteases, e.g. by considering where the peptides overlap and...

02 March 2015 3,712 3 View