I've had a look in Google Scholar and PubMed but I can't find anything useful so far.
As far as I know, many cytokines have quite short life times, which is also consisting with my in-vitro experiments with Mono-Mac-6 cells. I never determined the half-time, but for TNF-a I could find this publication (attached as link), where they could show a half-time of 18.2 minutes.
http://www.ncbi.nlm.nih.gov/pubmed/8324076
Hi Dogan,
I had a quick Google and found that publication as well. That publication seemed to be an in vitro experiment with whole blood, whereas I was hoping to find some info on recombinant cytokines.
I used to use it for overnight stimulations in HUVEC cells in culture, so I guess it is active for at least this long - don't know about the half life though. The stimulated phenotype in these cells was very obvious
Do you buy these recombinant cytokines? If yes, you could maybe ask the company, because it's really almost impossible to find such information about cytokines. I will keep on searching. It's also very interesting for me. Maybe you coould also share information, you will maybe get from Mr. Havenaar.
Dear Dogan Doruk Demircioglu,
I have searched for the article you mentioned on TNF half-life. In pubmed abstract is available but not the article. If you have the article, would you please attach it and send?
Hey Dogan,
I also tried looking and couldn't find the full article. Could you possibly send it or upload it.
Much appreciated.
You can find some answers from the paper: Kamada et al.,2000:Antitumor Activity of Tumor Necrosis Factor-α Conjugated with Polyvinylpyrrolidone on Solid Tumors in Mice.
Here is the link: http://cancerres.aacrjournals.org/content/60/22/6416.full
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