Hello Irena,

it is a normal response for fibroblasts. I do not know which specific cell line you are using, but most fibroblasts (MEF, MDF) only activate the NF-kappaB and MAP kinase signaling pathways and do not die upon single TNF administration. The proteins involved NF-kB/MAPK pathways actively repress cell death induction, so you need to inactivate one of these cell death checkpoints to start seeing cell death. Depending on the cell death checkpoint you affect, different proteins will regulate the induction of cell death. So you need to consider how you will induce cell death depending on your scientific question. I will refer you further to a recent review on this topic from our London colleagues:

Article Checkpoints in TNF-Induced Cell Death: Implications in Infla...

best,

Yves

Thank you for your response! Do you know if IFN-y would have an apoptotic effect?