Irena Muffels

3 Questions 3 Answers 0 Followers

Questions related from Irena Muffels

Is HOECHTS/PI staining (and then quantify) a good assay to determine the amount of dead cells of a whole population?

I want to treat fibroblasts with 24h MG132 and then look a the amount of cell death. However, I want to correct for the amount of cells in total, because they also prolfirate in 24hour. My...

14 November 2018 5,532 3 View

Celltrace Violet doesn't work on fibroblasts?

Currently, we are trying to measure proliferation speed in fibroblasts from healthy donors and patients. We are trying this with Celltrace violet. We tried different concentrations, 2 uM, 5 uM and...

15 October 2018 8,185 5 View

Does anyone know the effect of TNF-a on fibroblasts?

We incubated them with TNF-a for 16 hours (concentrations 0.125-5-20-80 ng/ml). However, none of them died/nothing happened. Has anyone ever conducted this experiment? We expected them to show at...

05 October 2018 1,643 2 View