You could but be aware that it may elute very close to your peaks depending on the column

The short answer is

briefly, NO if you intend to use toluene to correct also for recovery (i.e. as a surrogate compound)

MAYBE (with the correct GC conditions) if you will use toluene only as an injection standard

Hi Irena,

When you choose an internal standard it should be similar or behave similar as the compounds you want to analyze. So same boiling point range, similar structure, similar polarity. The best internal standards are deuterated or C13 standards of he compounds you want to measure. If you analyse compounds spread over a wide boiling point range you should use several internals standards. 

And of course the compounds should not be in your matrix. If you use toluene as a solvent on your lab then this could be a problem, there is a change you could pollute your sample.

Toluene will eluted before any of the monoterpenes, so maybe the boiling point is too low for being a good internal standard. It can be uses as an injection standard though.

I assume you will also use terpene standards? 

Regards, Alex

Skomantas Serapinas, Salvatore Chiavarini and Alex Van Renesse thank you so much for your responses. I will try to apply your advices.