Western blot with isopentane samples??

Hello, I have muscle tissue samples from mice that have been frozen in methylbutane (isopentane) for further cryostat cutting and histological analysis. However, my protein of interest does not...

15 September 2020 2,430 2 View

How much should/can I change parameters in a fluorescence microscope?

Hi everyone, I am performing some immunofluorescence in mice's muscle tissue. I am doing some antibodies concentration testing and the thing is: When I put my tissue under microscope, there is no...

05 August 2019 5,622 5 View

Does anyone know how to make Antifading homemade solution?

I need to prepare Antifading (like Citifluor) solution for FISH technique.

19 November 2016 1,983 2 View

Can anyone suggest a statistical method to detect differences in predicted mortality vs. actual mortality in highly variable mixtures testing data?

I am testing for differences in mortality from PAH mixtures based on predicted mortality from individual PAH exposures. Since single PAH testing had high variability in response between...

26 March 2013 2,775 7 View

Student experiences of Work Integrated Learning

If you are a current health science student (Nurse, Dr, Midwife, Paramedic, etc), I am keen to learn about your experiences of Work Integrated Learning (WIL). Reflective questions: What have been...

01 January 1970 3,956 5 View

Will you be evaluating microclimates within wilting and wilted flowers?

01 January 1970 7,201 23 View

How can I access SEGAE for my students of sustainable agriculture in Canada?

This seems like a great program. Can it be accessed by students outside of Europe? I'd love to add it as a component of a course that I teach at Trent University in Ontario, Canada.

01 January 1970 5,780 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View