Marlies Boeren

4 Questions 5 Answers 0 Followers

Questions related from Marlies Boeren

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

20 February 2021 8,613 2 View

How to combine 2 independent sample T tests with different group sizes in SPSS?

Hi, I carried out 2 experiments with the same experimental conditions Exp1 has 3 groups of 8 replicates Exp2 has 3 groups of 6 replicates Since I am interested in a dose-effect size, I did...

27 November 2020 7,668 3 View

Why do the axons of my hiPSC-derived neurons don't grow through the microgrooves of my compartmentalized chamber?

I am culturing human iPSC-derived neurons in compartmentilized chambers. I have run multiple experiments where axons grow nicely through the grooves. However neuronal cells from the same batch now...

04 March 2020 5,807 0 View

Does anyone know the composition of CultureOne supplement (ThermoFischer) or an alternative?

Since there is a shortage of the CultureOne supplement (ThermoFischer), I am looking for alternatives. I tried differentiation of NSC into neurons without the supplement but that doesn't look great.

09 April 2019 331 1 View