I'm evaluating several possibilities, from ELISA to HPLC but what really interests me is the best way to evaluate the state of the liver function of the zf larvae.
Hi Ignasi,
Maybe checking biochemical activity of acetiltransferase and transaminase homologs -like in humans-?
in humans is measured in blood, don't know exact protocol...
We are trying to compare results from a cell culture analysis. we have done 3 or 4 replicates per condition which is common practice. Student's t-test is often used in similar publications,...
04 July 2023 5,640 3 View
Hi everyone, I'm trying to develop an experiment in-vitro to determine that a molecule with phosphatase inhibition activity makes an action by inhibiting one or some phosphatases. Does anyone know...
30 April 2023 3,364 0 View
Hi everyone, I'm trying to define the MoA of a molecule that we already know inhibits some phosphatases by attaching them depending on the dose range. But we don't know if there is one or more...
27 April 2023 7,584 5 View
Hi eveyone, I'm trying to check whether there's some previous evidence of this fact, with particular interest in the Mediterranean. Thanks to a database, we have collected enough information to...
02 September 2018 2,465 3 View
Adaptive support programs have the risk to slow down students learning, especially if they forget non academic capabilities or they are subordinated to the previous knowledges. We are talking...
24 February 2017 1,869 0 View
Are long-lived species more resistant to extreme disturbances (i. e. heat waves, fires, hurricanes, etc) than shorter-lived species? If I’m right, longevity is driven by survival rates. Therefore,...
30 March 2015 3,093 2 View
I am comparing longevity using as a proxy simulations of maximum conditional lifespan based on published matrix models. Most of the transitions that I’m using are based on annual population...
30 March 2015 8,962 2 View
I am looking for published papers on demographic matrix models for different marine sessile species. So far I have been able to find several studies conducted in algae, corals and gorgonians but I...
05 December 2014 8,774 1 View
I would appreciate any reference of publications containing good definitions for settlement and recruitment in sessile organisms.
16 October 2014 5,239 4 View
I would like to know how many pixels of the scanned flat skin is of every color in order to compare between species. In particular we are studying the color skin differences between Apodemus...
23 May 2013 9,624 16 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
zebrafish and mouse
19 July 2024 9,158 1 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...
25 June 2024 4,602 0 View
Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?
13 June 2024 389 0 View
I want to teach a class using zebrafish embryos. It's in the afternoon, so I want to get my hands on some fake, reusable embryos that students can use for practice. Does anyone know of any?
10 June 2024 3,956 1 View
I did comet assay with goldfish in my master's degree but now I am experiencing such a problem. has anyone encountered such a problem before? the only difference from my other experiments is that...
09 May 2024 6,902 3 View
Hi all, suggestion for antibodies labelling proliferation that work well on zebrafish tissue? I ve used pH3 but I want something labelling more than just one phase (or labelling longer phases such...
25 April 2024 7,182 3 View
Hello everyone, I would like to know whether we can use the vibratome to section zebrafish larvae (8-9 days post fertilization) for histology. We have a Leica VT1200S vibratome in our lab....
15 April 2024 1,994 0 View
Hi all, I'm trying to isolate zebrafish embryonic stem cells after 3 hpf of zebrafish embryo's. I have a couple of questions. - I already tried Pronase to dechorionate, but it is very difficult....
15 April 2024 9,059 0 View