I need Coccothrinax readii seeds, where can I find it?, and when is their fructification season? Can someone help me?
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
29 July 2024 6,350 3 View
how do i set one up? What do I include?
22 July 2024 6,097 4 View
I am currently using a map downloaded from https://gadm.org/maps/DEU/bayern.html and I would like to assign the value of a variable to each cell with R. I was also trying to get this information...
15 July 2024 8,844 1 View
I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...
09 July 2024 4,857 2 View
I’m currently working on polyglutamine disease by Sanger sequencing to count the CAG repeats. However, since my targeted genes involve CAT or CAA interruption in the CAG repeats, their...
01 July 2024 8,328 5 View
I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.
22 June 2024 4,859 8 View
Hello, I am making lipid nanoparticles using the thin film hydration method. Formula is DODMA:DSPC:Cholesterol:DSG-PEG2000 at 60:20:17.5:2.5 %mM in 5mM total. After hydration in sodium acetate...
16 June 2024 6,842 6 View
Taking into account that each society incorporates into the curricula the necessary knowledge to teach depending on the social demands of each country.
06 June 2024 9,710 14 View
Biodiversity assessment of river ecosystems is crucial for understanding the health and stability of these environments. This question aims to explore the various techniques employed to evaluate...
07 August 2024 4,290 3 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.
11 July 2024 9,045 4 View
The question refer to leafy biomass decomposition using litterbag techniques.
11 July 2024 2,078 0 View
I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...
10 July 2024 7,145 2 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View