Hi Lykke

From former studies it is known that ammonia builds up in muscle as a consequence of amino acid catabolism http://www.ncbi.nlm.nih.gov/pubmed/1581847

Perhaps you could try to measure this? Also, it is known that BCAA are a major source of deaminated AAs. Perhaps you could measure the expression of BCAT 1 or 2? The BCKDH is also regulated by phosphorylation similar to PDH and perhaps this also happens during exercise and is differentially regulated in your mice. I believe that you could either collaborate to get abs or there are perhaps commercial vendors.

There is also a good paper on this in mice: http://www.ncbi.nlm.nih.gov/pubmed/20133434

Good luck!

Adam

Thank you very much for this Adam!! I very much appreciate it and I will try to look into the option of analyzing ammonium or BCATs, although the latter may not be descriptive of what happens during exercise. However, it looks like it is actually not that easy to analyze brotein breakdown/utilization in muscle following exercise. -at least not on muscle lysate.

Thanks again!

Best wishes,

Lykke