How can I measure prenylations on an IP of my protein?

19 December 2014 1 8K Report

I want to measure prenylations, specifically geranylation. I have a FLAG epitope on my protein and want to do an IP and then blot for geranylations. However, I cannot find any geranylation antibodies. -does anyone know of usable antibodies?

Kai F. Albring

Hi Lykke,

if are using a vector with some viral promotor like CMV to overexpress your FLAG-tagged protein of interest, you will most probably overwhelm the cellular machinery involved geranylation. Some of the protein might still be modified but it could be that the majority is not.

If possible, you should try to do an IP of endogenous protein. This approach should yield more reliable information than trying to analyse modifications on overexpressed proteins.

Best,

Kai

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