Dear David,

Since TRP and CaR channels are tightly interactive, both compounds, (i) anionic  (such as EETs) together with (ii) cationic (gadolinium, spermine, Li, Na, Mg, etc)  are highly involved in TRP-channel function.

Yes, for vasodilation and vasoconstriction an epoxyeicosatrienoic acid and derivatives are important via TRP regulation, but polyamines can be also involved since TRP channels can be agonized by organic cations such as polyamines,  spermine (SPM), spermidine and putersine (Ahern et al.,2006, see attached) and that may be a common for TRP family (TRPC, TRPM, etc.).

Since in brain glio-vascular interface (blood-brain-barrier) requires glial cell (astrocytes, Mueller cells) participation,  Eric Newman described the EET effects (attached). this attarcts a role of glial cells which regulate blood circulation in brain and retina. Another chemicals such as N-methyl maleimide or allyl isothiocyanate can do so as well. Also a deactivation and desensitization kinetic can be different.

I want to bring your attention to polyamines because in brain and retina these compound are almost exclusively accumulated in glial cells (Laube and Veh, 1997; Biedermann et al, 1998, attached).

I wish you great success and cordially, Serguei.

Thank you for your answer Serguei. Definitely food for thought.