Does anyone know of a resource detailing RNA expression in wild type HEK 293 cells?

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Maximilian Peters

Did you try to find your transcription factors in the GEO datasets on PubMed?

Kristopher R Bosse

Thanks, I am interested in looking at it from the side of 293 cells- but I will take a look at it from theat end too.

Daniel M Cohen

Kristopher- it's not a perfect solution, but ENCODE has deposited Exon Array data and PolII mapping for WT 293 as well as 293T cells. Can give you a feel for whether a particular transcription factor is expressed at the mRNA level or not in this cell type. You can view these data on UCSC browser in the hg19 genome.

Thomas K Albert

Hi Kristopher, there are currently 1333 hits in GEO DataSets if you search for HEK293; numerous contain "wildtype" 293's, e.g. series GSE1676...

And I agree with Maximilian that it is probably a good idea to look from the Tx factor direction into these GSE sets, otherwise you might get lost in the wealth of data...