Ideally, this would be an antibody that did not also detect phospho-TrkA or phospho-TrkB and would detect the protein in rhesus/human cell extracts by western blot.
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.
25 July 2024 9,976 3 View
I have seen two kinds of explanations given for the phenomenon that I try to draw in the attached png document. One explanation says that an air bubble creates the experience of a solid black...
16 June 2024 6,493 1 View
Cross-slip, twinning and fracture are major deformation modes adopted by loaded materials. It appears sound that these apparently different deformation mechanisms can be analysed on the equal manner!
15 June 2024 8,477 3 View
The terms Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction has been used interchangeably in papers that I have read. Are there differences in these...
05 June 2024 3,453 7 View
Investigating use of Sorghum as part of Regenerative Agriculture management system using cover crops & mower-crimping, Alley Cropping, sheep manure in a zero till management system with...
18 April 2024 2,389 3 View
I'm conducting a multiple case study, and I'm interested in exploring a phenomenon from the perspectives of male and female participants.
14 April 2024 4,352 1 View
I need to prepare Na2HPO4/Citrate buffer pH 5.0, it is possible to prepare 1M, 0r 0.5M if yes, please how? Thank you I want to crystallize my protein with this buffer? i need 0.1M working...
03 April 2024 10,006 0 View
The following are the details of the conference: Name of event: International Conference on Community Health Nursing and Public Health (ICCHNPH) Venue: Brno, Czech Republic Date: 9th -10th...
25 March 2024 996 1 View
For cell culture , if want to study about the protein or gene expression ? The treatment need to dilute with medium without fbs & pen strep or complete medium. What happen , if the treatment...
19 March 2024 399 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
I am working on flourescence sensing of heavy metals using quantum dots.Can any of you suggest how to find the detection limit. Whether it is calculated from formula or we can get from instrument?
19 July 2024 7,411 3 View