We want to use a chimera of DNA binding protein (to a specific DNA sequence) and strepavidin. Do you know of any from the literature or personal experience?
Malcolm, while I have no experience with streptavidin-DBD fusions I do have experience creating a fusion between a chromatin remodeling protein (Chd1) and streptavidin.
(if interested, see https://academic.oup.com/nar/article/41/3/1637/2903312)
We needed to disrupt the tetramer interface to get this to work properly for our work (see http://www.jbc.org/content/280/24/23225.long). The monomeric streptavidin was better-behaved for our purposes. I don't imagine much difficulty creating a fusion to a DNA binding domain - do you have anything specific you want to know about such fusion proteins? To be honest, I've been meaning to create some DBD-streptavidin fusions but haven't had the time yet, so I certainly think it's a good idea. Feel free to follow up privately if you have any questions I can help you with regarding this topic.
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