A. Malcolm Campbell

7 Questions 12 Answers 0 Followers

Questions related from A. Malcolm Campbell

How can I remove oil from droplets formed by microfluidics?

I want to move into using nano-scale aqueous droplets to conduct biochemical reactions. Anyone have good ways to remove the oil after reaction is completed?

29 April 2019 8,283 4 View

Causes of high variation in RFP production in E. coli?

For years we have seen variation in RFP produced in E. coli. We have used different promoters and RBSs, but always the same RFP gene (see this as an example:...

11 July 2018 1,318 6 View

Does anyone know of a DNA-binding domain + strepavidin fusion protein?

We want to use a chimera of DNA binding protein (to a specific DNA sequence) and strepavidin. Do you know of any from the literature or personal experience?

30 May 2018 5,737 1 View

Thinking about starting some experiments using chemostat. Is this something I can buy, or do we need to build using components?

We will be working with E. coli. We do not have graduate students or postdocs, so we are looking for an easy system to get up and running without a lot of customization. We need 37 C growth...

19 May 2016 8,686 2 View

Do you have a commercial source for outsourcing DNA sequencing?

I want to send out a 96 well plate containing bacterial cultures and have all 96 mini-prepped and sequenced by the company. Does anyone know of a commercial source for this and possible pricing?

12 March 2014 5,192 7 View

We have a plasmid that contains kanamycin resistance but it allows E. coli to grow on ampicillin as well as kanamycin.

Have others seen this too? Any suggestions how to fix it?

11 February 2013 6,106 17 View

Need Ori DNA Sequence

I am looking for the DNA sequence of a "run away plasmid" origin of replication that comes from a plasmid called pKN402. The Ori is in the family of replicons called IncFII or pT181 that represent...

20 January 2012 3,651 2 View