Does anyone know of a database where I can find information on alternative 3'UTR length of gene transcripts in different human brain regions?

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Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...

29 July 2024 4,630 0 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

I need help with cloning a 200 bp insert and a 4,7 Kb pEGFP-N1 vector. How can I do it?

I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...

09 July 2024 4,277 1 View

El programa Eisanalyser de ajuste de circuitos ya no abre para Windows 11 y 10, ¿que puedo para que funcione de nuevo?

Saludos a todos. Recientemente he tenido inconvenientes con el programa de ajuste de circuitos Eisanalyser ya no abre para Windows 11 y 10, hace unas semanas funcionaba bien y dé repente dejó de...

21 April 2024 7,798 1 View

Até que ponto as tecnologias digitais prejudicam ou ajudam no aprendizado das crianças? Como usar as tecnologias digitais na educação infantil?

Espera-se encontrar artigos que abordem meios de inserir as tecnologias digitais nas práticas pedagógicas da Educação Infantil de forma saudável, controlada e segura. Trabalhos bibliográficos ou...

15 April 2024 6,789 0 View

Modeling ods 8 with ode?

Hello, I've been scouring the internet for a scientific article that models Sustainable Development Goal 8 (Decent Work and Economic Growth) using Ordinary Differential Equations (ODE), but I...

30 March 2024 8,477 3 View

¿Cómo se implementaría una metodología participativa que involucre a personas mayores en el diseño y desarrollo de programas de bienestar Social?

Nos enfocamos en como diseñar estrategias participativas para involucrar a los mayores, y lo más importante es que respete su autonomía y dignidad. Buscamos entender como incluirlos en la toma de...

10 March 2024 2,659 3 View

Visualization of viruses with TEM with formvar-only coated grids?

Hello, I have available TEM copper grids coated with Formvar but not carbon. I would like to visualize Viruses in suspension from a concentrated sample. Would the lack of carbon coating affect my...

06 March 2024 1,219 0 View

Plasma cleaner for glow discharge?

Hello, I have available a Solarus II Plasma Cleaner. Would plasma cleaning glow discharge formvar coated TEM copper grids? I want to visualize viruses in suspension. Thank you, Paula

06 March 2024 7,453 0 View

What could this additional band be?

Hello! Recently, I ordered an FOLH1 ORF, which came inserted into the pUC-57 mini vector. Additionally, I requested BamHI and XbaI restriction sites to be included at each end of the ORF. When I...

25 February 2024 7,328 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View