Majid Kamli

3 Questions 11 Answers 0 Followers

Questions related from Majid Kamli

Does anyone have experience to Clone oligos into pLKO.1 neo - based lentiviral vector (Addgene) for shRNA Constructs ?

I cloned my interested shRNA sequence in pLKO.1 neo - based lentiviral vector between Agel and ECOR1. After ligation when I did restriction digestion I got three bands with Nco1 and ECOR1...

04 July 2015 4,860 3 View

Can anyone suggest an assay to check the impact of point mutation in promoter site?

I have gene having two variants (SNP). The case is compound heterozygous i.e one variant is maternal and other is paternal. One variant is on exon and another is on promoter site of the gene....

19 June 2014 8,821 3 View

Can anyone suggest a tool by which I can locate non coding RNA for a particular gene?

13 March 2014 7,746 1 View