Hi Bartlomiec,

I have worked with mice, rat and rabbit dark-adapted whole-mount retinas. It will be very difficult and time-consuming to patch cells (eg, ganglion cells) with an inverted microscope. It will be hard to find, visualize and drive the electrode through the tissue onto the target cell on the other side of a thick whole-mount tissue of 300-400 microns. Patch electrodes require a clean tip and clean cellular membrane. Therefore if you must, then I would recommend to try sharp electrodes, which are easier to drive through thick tissues without significant blockage of the tip. Since the tip is only a few 100 nanometers, you may not be able to view but just do blind sharp electrode recordings at the right layer or depth of the tissue.

best wishes,

Refik

Dear Sir: The upright microscope is better microscop