I will perform multiple omic analyses in a sample, but for redox proteomics an akylating agent needs to added in the lysis solution (iodoacetamide or NEM). The problem is that the sample will be splitted in two parts, half for redox proteomics, and half for metabolomics and lipidomics.

The aqueous phase of half of the sample will be used used for metabolomics (and it will be contaminated with iodoacetamide). So I have to remove it befor metabolomics, and the workflow includes HILIC separation. So, I would like to know if by using the HILIC column it will be enough to remove iodoacetamide, or if iodoacetamide will interact with the column and disturb the analisys.

Or still, if anyone can think in another way to remove this iodoacetamide from the aqueous phase that will be used for metabolomics. Remembering that for metabolomics small MW molecules are analyzed.

I need to use the same sample for all analyses, because this is the type of approach that we intend with this project.

Thank you all in advances!