I am working on a plant with high polysaccharide content. For genomic DNA extraction, we have used several protocols but failed to get a good quality DNA. We found out that the STE/CTAB approach might be useful (Shepherd & McLay, 2011). For preparing STE the following materials are needed (0.25 M sucrose, 0.03 M Tris, 0.05 M EDTA). However, we still do not know the amount (gr/mg) of the material we should take to prepare STE. Does anyone have any experience?