Do mature (virgin) forests take up carbon, if there is no net increase in biomass?

I seem able to get any answer I please just by searching a little more. Where is the truth? Concerning untouched Amazon rain forest, when people measure carbon flux, do they include the carbon...

27 October 2020 2,546 4 View

If one wants to link together power sources all across North America, would it be better to use DC transmission lines, or gas pipelines, say with H2?

Intermittent renewable energy sources become more efficient and reliable as the number and diversity of sources is increased. But electrical transmission over distances greater than 2000 miles...

20 October 2020 731 5 View

Using antimicotics such as Nystatin or amphotericin B in mouse neuronal cultures?

Hi all, I just started to prep mouse cortical neutrons for E16.5 and in my second prep I had a massive fungal contamination. Since these preps are quite precious, I wonder if I could add an...

15 September 2020 645 1 View

Is it possible to add a selection agent in using pCAGGS for transfection of HEK-293T cells?

I will try to express proteins in HEK-293T cells. I had previous experience in another cell line where I used co-transfection for selecting transfected cells. I had observed lower levels of...

16 May 2020 2,857 1 View

Does anyone have a general Hydrophobic Interaction Chromatography Protocol?

I have a sample suspended in 0.34 M NaCl after using ion exchange chromatography. I would like to perform hydrophobic interaction chromatography (HIC). Is it okay that I use greater salt...

27 August 2019 8,417 1 View

What is the difference between the columns Hi Prep 26/60 S-100 HR and Hi Prep 26/60 S-200 HR?

I am trying to have a better resolution during purification. Would running my sample using S-100 HR give a better result from an S-200 HR run? Also, other than the fractionation range, what is...

24 July 2019 4,378 3 View

Exemplary models of elementary school speaking-listening skills group instruction-assessment: Successful school sites and teachers?

School districts make public claims and governmental contracts regarding essential English Language Arts Speaking skills, however transparency and accountability is lacking about implementation....

22 January 2019 2,420 3 View

For EV development, what is a good number for battery energy density?

For future EV design, I have tentatively chosen 0.157 kWh per kg. for energy density of the battery pack. Am I aiming too low for a vehicle likely to reach production in five years? Or, is my...

27 December 2018 2,368 1 View

Why the pH of xanthan gum in aqueous solution is 7 (neutral) when it has glucuronic and pyruvic acids in its chemical structure?

I find it pretty confusing since xanthan gum has both glucuronic and pyruvic acids in its chemical structure, it should release H+ ions upon solvation in an aqueous solution and make it acidic.

18 January 2018 9,190 2 View

Does anyone observe decrease in growth rate of Drosophila S2 cells in serum-free media?

I have adapted my Drosophila S2 cells growing in complete Schneider's medium to serum-free media. After a month of passage in serum-free media, the cells continually exhibit decreased growth rate...

04 December 2017 9,887 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View