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Questions related from Ernest Troyss Pilapil
I will try to express proteins in HEK-293T cells. I had previous experience in another cell line where I used co-transfection for selecting transfected cells. I had observed lower levels of...
17 May 2020 3,009 1 View
I have a sample suspended in 0.34 M NaCl after using ion exchange chromatography. I would like to perform hydrophobic interaction chromatography (HIC). Is it okay that I use greater salt...
28 August 2019 8,549 1 View
I am trying to have a better resolution during purification. Would running my sample using S-100 HR give a better result from an S-200 HR run? Also, other than the fractionation range, what is...
25 July 2019 4,529 3 View
I have adapted my Drosophila S2 cells growing in complete Schneider's medium to serum-free media. After a month of passage in serum-free media, the cells continually exhibit decreased growth rate...
05 December 2017 10,011 2 View
I did a western blot with a PVDF membrane. Because I only have 5 samples, I have thought of using 5 ml (1:1000 antibody dilution) of antibodies (Ni-NTA AP Conjugate). Unfortunately, I have...
12 July 2017 1,488 2 View
I am about to reconstitute a Schneider's powdered medium from Sigma. I have read their protocol that says in step 10, osmotic pressure of 360 mOsm is suitable for the growth of the Drosophila...
11 July 2017 8,248 2 View
I will be using the reagent for extraction purposes
22 August 2016 9,169 6 View
