Few years ago Bayer had glycated hemoglobin strips for human (A1C Now+) that perfectly measured glycated hemoglobin in rats, but those strips were discontinuated. Then we started to use a DCA System from Siemens, but the detection range doesn't allow us to differenciate glycated hemoglobin, namely between diabetic rats of the control group and diabetic rats of the therapy group. Does anyone know or use any other measurement assay with good detection range in rats? We are also interested in Fructosamine assays, but it will be helpful if there was no need to do colorimetric/ELISA assays, and that's why those strips from Bayer were an excellent option.
Thank you!!
Ana
Simple method for determination of A1c-type glycated hemoglobin(s) in rats using high performance liquid chromatography.
Kondo N1, Shibayama Y, Toyomaki Y, Yamamoto M, Ohara H, Nakano K, Ienaga K.
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Abstract
We have developed a new chromatographic method for the determination of rat glycated hemoglobins by cation exchange high-performance liquid chromatography. The hemoglobins were eluted by a two-step gradient, and the total assay time, including re-equilibration of the column, was 27 min. Two A1c type and one pre-A1c type rat glycated hemoglobins were separated and measured. The change in major HbA1c of rats, in which diabetes was induced by streptozotocin and which were subsequently treated with insulin, was monitored. In diabetic rats (n = 10, average blood glucose greater than 300 mg/dL), major HbA1c rose to 3.39 +/- 0.06% compared with controls (n = 10, 1.20 +/- 0.03%) during 5 wk. Insulin treatment decreased the HbA1c from 3.48 +/- 0.16% to 2.74 +/- 0.15% (p less than 0.01) in 6 wk. This method was also effective for the determination of mouse HbA1c.
PMID:
2724990
[PubMed - indexed for MEDLINE]
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