I need a easier and cheaper method than HPLC analysis like chemical analysis or spectroscopic method to determine the levels of glycosid steviols in Stevia rebaudiana extracts.
For qualitative and quantitative analysis of any herbal extract for phyto-chemical HPTLC is very dependable and faster technique than many others. For Steviol glycosides please refer..... 2 articles given below
1. Evaluation of steviol and its glycosides in< i> Stevia rebaudiana leaves and commercial sweetener by ultra-high-performance liquid chromatography-mass …
C Gardana, M Scaglianti, P Simonetti - Journal of Chromatography A, 2010 - Elsevier
... was also proposed to evaluate steviol-glycosides [23], together with a validated HPTLC procedure
with ... detection [24] and a NIRS procedure for the quantification of steviol-glycosides [25 ... Recently,
2. a semi-quantitative determination of ST-glycosides was performed by desorption
Validated high-performance thin-layer chromatography method for steviol glycosides in< i> Stevia rebaudiana
V Jaitak, AP Gupta, VK Kaul, PS Ahuja - Journal of pharmaceutical and …, 2008 - Elsevier
... In the present study an HPTLC method was developed for the quantification of steviolbioside,
stevioside and rebaudioside ... is simple, cost effective, eco-friendly and easily adaptable for
simultaneous screening and quantitative determination of steviol glycosides as compared ...
HPTLC is alternative technique read following paper
J Pharm Biomed Anal. 2008 Aug 5;47(4-5):790-4. doi: 10.1016/j.jpba.2008.03.022. Epub 2008 Mar 28.
Validated high-performance thin-layer chromatography method for steviol glycosides in Stevia rebaudiana.
Jaitak V, Gupta AP, Kaul VK, Ahuja PS.
Stevia (Madhu Patra) – A natural low calorie sweetener with more promising benefits
BM Sattigeri
INTERNATIONAL JOURNAL OF PHYTOTHEARPY RESEARCH 2012, 2(3):20-25.
http://earthjournals.org/ijpr_117.pdf
Yes actually the HPLC method is most common and complete way to analysis and determine the stevia glycosides, but for some reasons we are not able to use the HPLC in lab, so i m going to find a replacement method to determine the levels of stevia glycosides in extracts
What instrument facility available at your side so we can suggest according to that
Best and economical way is......
1. Understand the research papers on HPTLC given above for HPTLC of "steviol glycosides in stevia rebaudiana extracts"
2. Try to develop small TLC plates (Silicagel 254 G.) and preserve the developed plates for scanning under CAMAG HPTLC scanner. (There must be one or two R&D Lab in Iran with HPTLC scanners and request them for some cooperation to scan your plates)
3.You may contact CAMAG distributor in IRAN ( as given in their sites.... M/s MAHZAD KALA CO. LTD.
142 Ostad Motahari Avenue Shahid Akbari Cross Road Bank Melli Bldg
IR-15787 Tehran Postal Code: 1578743338 Telephone+98 21 888 46182-4 Fax
+98 21 888 46185 [email protected] www.mahzadkala.com)
This way you can quickly and with less cost analysis.Best of luck
if you want to quantify glycosides, I suggest HPLC, with an optimal extraction of the leaves, however if you want only to detect glycosides, you can use thin layer chromatography TLC.
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